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KMID : 1377020140110030203
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Tissue Engineering and Regenerative Medicine
2014 Volume.11 No. 3 p.203 ~ p.210
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Silk fibroin based hydrogel for regeneration of burn induced wounds
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Ju Hyung-Woo
Lee Ok-Joo
Moon Bo-Mi
Faheem A Sheikh
Lee Jung-Min
Kim Jung-Ho
Park Hyun-Jung
Kim Dong-Wook
Lee Min-Chae
Kim Soo-Hyeon
Park Chan-Hum
Lee Hae-Ran
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Abstract
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The present study deals with fabrication of hydrogel composed of silk fibroin, calcium alginate and carboxymethyl cellulose. A simple mixing of calcium alginate and carboxymethyl cellulose and silk fibroin was adopted to fabricate new silk fibroin based hydrogel (SFH) type. The adhesive strength, water content and cytotoxicity of fabricated hydrogel was comparatively investigated against standard control as medical gauze (C) and Purilon Gel® (PG). The results from the adhesive strength indicated that presented hydrogels had least adhesive strength but had good cell viability than the C and PG. The cell toxicity results after 1 day of culturing NIH 3T3 fibroblasts indicated that prepared hydrogels comparably promoted cellular growth. The in vivo experiments using Sprague-Dawley were done by creating a second-degree burn on the back of rats to study the effect of healing process. The gross examination investigations at 1, 3, 7, 14 and 21 days indicated a progressive effect induced by the SFH. The histological examinations using Hematoxylin and eosin (H&E) and Masson’s trichrome (MT) staining after 3, 7, 14 and 21 days indicated the complete collagen deposition and dermic formation using the presented SFH. Our results suggest that SFH can be considered as promising new candidate matrices for burn wound regeneration.
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KEYWORD
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silk fibroin, hydrogel, burn, dressing, biocompatible
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